|
KMID : 0043320120350101811
|
|
Archives of Pharmacal Research
2012 Volume.35 No. 10 p.1811 ~ p.1816
|
|
|
Slow-binding inhibition of soybean lipoxygenase-1 by luteolin
|
|
Ha Tae-Joung
Lee Myoung-Hee
Kim Hyun-Tae
Kwon Hyun-Sook
Baek In-Youl
Kubo Isao
Jang Dae-Sik
|
|
|
Abstract
|
|
|
|
Luteolin, isolated from the seeds of Perilla frutescens (perilla seeds), inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC50 of 5.0 M (1.43 μg/mL) noncompetitively. The progress curves for an enzyme reaction indicate that luteolin shows slow binding kinetics. Both the initial velocity and steady-state rate in the progress curve were decreased with increasing the concentration of luteolin. The kinetic parameters, which described the inhibition by luteolin, were evaluated by nonlinear regression fits.
|
|
|
KEYWORD
|
|
|
Lipoxygenase-1, Luteolin, Perilla frutescens, Slow-binding inhibitor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|